Response of rat tracheal epithelium to ozone and oxygen exposure in vitro
Identifieur interne : 001C09 ( Main/Exploration ); précédent : 001C08; suivant : 001C10Response of rat tracheal epithelium to ozone and oxygen exposure in vitro
Auteurs : Kristen J. Nikula [États-Unis] ; Dennis W. Wilson [États-Unis]Source :
- Fundamental and Applied Toxicology [ 0272-0590 ] ; 1990.
English descriptors
- Teeft :
- Amer, Basal, Basal cells, Carbon dioxide, Cell damage, Cell population, Cilium, Columnar, Culture medium, Dioxide, Epithelial, Epithelial injury, Epithelium, Intermediate cells, Metaplasia, Metaplastic, Metaplastic cells, Morphologic, Nikula, Nonciliated, Organ culture, Oxidant, Oxygen exposure, Ozone, Ozone exposure, Preciliated cells, Predominant cell types, Pseudostratified, Respiratory epithelium, Secretory, Secretory cells, Secretory granules, Serous, Serous cells, Sigler, Sloughed, Sloughed cells, Squamous metaplasia, Statistical significance level, Trachea, Tracheal, Tracheal epithelium, Tracheal organ cultures, Ultrastructural, Ultrastructural study, Vivo.
Abstract
Abstract: Although ozone-induced epithelial injury in vivo has been morphologically characterized, effects of gaseous oxidants on respiratory epithelium in organ culture, where tissue organization is maintained but systemic influences are eliminated, have not been thoroughly investigated. In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed in vivo occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. We conclude that there was a synergism between oxygen and ozone exposure leading to enhanced epithelial injury and metaplasia.
Url:
DOI: 10.1016/0272-0590(90)90169-K
Affiliations:
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Nikula</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Nouveau-Mexique</region></placeName><wicri:cityArea>Inhalation Toxicology Research Institute, Lovelace Biomedical and Environmental Research Institute, P.O. Box 5890, Albuquerque</wicri:cityArea></affiliation></author><author><name sortKey="Wilson, Dennis W" sort="Wilson, Dennis W" uniqKey="Wilson D" first="Dennis W." last="Wilson">Dennis W. Wilson</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Pathology, School of Veterinary Medicine, University of California, Davis</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Fundamental and Applied Toxicology</title><title level="j" type="abbrev">YFAAT</title><idno type="ISSN">0272-0590</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990">1990</date><biblScope unit="volume">15</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="121">121</biblScope><biblScope unit="page" to="131">131</biblScope></imprint><idno type="ISSN">0272-0590</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0272-0590</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amer</term><term>Basal</term><term>Basal cells</term><term>Carbon dioxide</term><term>Cell damage</term><term>Cell population</term><term>Cilium</term><term>Columnar</term><term>Culture medium</term><term>Dioxide</term><term>Epithelial</term><term>Epithelial injury</term><term>Epithelium</term><term>Intermediate cells</term><term>Metaplasia</term><term>Metaplastic</term><term>Metaplastic cells</term><term>Morphologic</term><term>Nikula</term><term>Nonciliated</term><term>Organ culture</term><term>Oxidant</term><term>Oxygen exposure</term><term>Ozone</term><term>Ozone exposure</term><term>Preciliated cells</term><term>Predominant cell types</term><term>Pseudostratified</term><term>Respiratory epithelium</term><term>Secretory</term><term>Secretory cells</term><term>Secretory granules</term><term>Serous</term><term>Serous cells</term><term>Sigler</term><term>Sloughed</term><term>Sloughed cells</term><term>Squamous metaplasia</term><term>Statistical significance level</term><term>Trachea</term><term>Tracheal</term><term>Tracheal epithelium</term><term>Tracheal organ cultures</term><term>Ultrastructural</term><term>Ultrastructural study</term><term>Vivo</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Although ozone-induced epithelial injury in vivo has been morphologically characterized, effects of gaseous oxidants on respiratory epithelium in organ culture, where tissue organization is maintained but systemic influences are eliminated, have not been thoroughly investigated. In this study, we exposed tracheal organ cultures from rats to 95% oxygen and 1 ppm ozone, alone and in combination, to determine (1) whether epithelial responses to ozone similar to those observed in vivo occur in airways separated from systemic physiologic, secretory, and inflammatory reactions; (2) whether concentrations of oxygen sufficient to potentially cause oxidant injury result in morphologic epithelial alterations similar to those that occur in ozone toxicity; and (3) if the combined oxidant insult of oxygen and ozone results in more severe damage to the tracheal epithelium than occurs with ozone in air. Tracheal organ cultures were exposed to filtered air and 5% carbon dioxide; filtered air, 5% carbon dioxide, and 1 ppm ozone; 95% oxygen and 5% carbon dioxide; or 95% oxygen, 5% carbon dioxide, and 1 ppm ozone for 96 hr. Light- and quantitative electron-microscopic evaluation showed that epithelia to 1 ppm ozone in air exhibited loss of ciliated cells and ciliated cell damage. The epithelia exposed to 95% oxygen and 5% carbon dioxide were pseudostratified, columnar, ciliated, and hyperplastic. Epithelia exposed to 95% oxygen plus 1 ppm ozone were stratified and nonciliated or very sparsely ciliated. The predominant cell types in epithelia exposed to oxygen plus ozone were serous cells and metaplastic cells, and focal aggregates of adherent necrotic cells were present. We conclude that there was a synergism between oxygen and ozone exposure leading to enhanced epithelial injury and metaplasia.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Nouveau-Mexique</li></region></list><tree><country name="États-Unis"><region name="Nouveau-Mexique"><name sortKey="Nikula, Kristen J" sort="Nikula, Kristen J" uniqKey="Nikula K" first="Kristen J." last="Nikula">Kristen J. Nikula</name></region><name sortKey="Wilson, Dennis W" sort="Wilson, Dennis W" uniqKey="Wilson D" first="Dennis W." last="Wilson">Dennis W. Wilson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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